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Updated: Apr 22, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
A reference genome assembly of Pteropus pselaphon
Kei Nabeshima1, Yasuhito Shimada2,3, Manabu Onuma4
1Biodiversity Division, Biodiversity Resource Conservation Office, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan. nabeshima.kei@nies.go.jp.
Abstract:
The Bonin flying fox (Pteropus pselaphon) is an endemic species of the Ogasawara Islands, Japan, and is classified as Critically Endangered. Here, we report a genome assembly of P. pselaphon, constructed using Nanopore long reads and Omni-C scaffolding. The final assembly spans 2.16 Gb across 929 scaffolds and achieves a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness score of C:98.2% [S:94.3%, D:4.0%], F:0.7%, M:1.0%. Gene prediction with Helixer software identified 20,647 protein-coding sequences, with a BUSCO score of C:92.2% [S:88.9%, D:3.3%], F:3.3%, M:4.5%. This genome provides a valuable resource for the conservation management of this endangered species, as well as for evolutionary genomics and studies of host-pathogen interactions in flying fox.
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