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Updated: Apr 22, 2026
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
Published on: July 27, 2022
Characterization of the Intestinal Metabolic Profiles Following Photon FLASH Irradiation
Siqian Zhang1, Qiliang Peng2, Jun Zhang1
1Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Objective:
Ultra-high dose rate radiotherapy, commonly referred to as FLASH radiotherapy, is a transformative focus in radiotherapeutic research. This study aimed to identify intestinal contents based metabolic biomarkers of photon FLASH.
Methods:
C57BL/6J mice were exposed to 0 Gy, 10 Gy, 12 Gy, or 14 Gy of photon FLASH irradiation (FLASH-IR) without a conventional irradiation. The intestinal contents were collected 3.5 d post-iiradiation, and subjected to liquid chromatography-mass spectrometry.
Results:
Metabolomic profiling revealed 25 differential metabolites that were consistently differentially regulated across the comparisons of 10 Gy vs. 0 Gy, 12 Gy vs. 0 Gy, and 14 Gy vs. 0 Gy groups. Notably, a dose-dependent positive correlation was observed for 3-hydroxyanthranilate, while 13 metabolites-including guanosine, adenosine monophosphate, and hydroquinone-exhibited a significant negative correlation with increasing FLASH-IR dose. Further enrichment analysis identified several perturbed metabolic cascades, prominently involving the PI3K-AKT signaling pathway, tyrosine metabolism, and purine metabolism.
Conclusions:
These findings illuminated the intestinal metabolic signatures post photon FLASH-IR, offering insights to optimize FLASH radiotherapy parameters and enhance its therapeutic ratio.
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