TissuStamp: a novel high-throughput tissue transfer workflow for FFPE spatial biology assays

Spatial biology is shifting from single-sample studies to cohort-scale analyses required for translational research, AI model development, and clinical deployment, yet tissue preparation methods have remained largely unchanged for decades, creating a critical throughput bottleneck. Existing approaches face a trade-off: traditional processing accommodates only a few samples per slide, while tissue microarrays increase sample count by restricting analysis to small cores that limit spatial context. This technical note describes TissuStamp™, a tissue transfer system that enables high-throughput FFPE tissue preparation for spatial multiomics. FFPE sections are first mounted on gel pads, then specific regions of interest are punched using square blades and 'stamped' onto surface-functionalized TissuGrip™ slides using a pusher block. This enables the transfer of up to 32 millimeter-scale tissue regions or 10 larger regions (totaling up to ~1,000 mm² of tissue area) onto a single standard 75 mm × 25 mm slide in a tightly packed, multi-lane array format. TissuStamp allows tissue cutting, storage and transfer to occur at different times and locations, facilitating multi-institutional studies and biobank initiatives. While TissuStamp was developed for the G4X™ platform, which performs integrated spatial transcriptomic, proteomic, and fluorescent H&E (fH&E™) profiling on up to 128 samples per run at subcellular resolution, its capabilities are transferable to other tissue analysis workflows. Comparative analysis demonstrated that TissuStamp preserved tissue morphology and delivered equivalent multiomic assay performance compared to conventional direct mounting onto slides. This method provides a practical solution for scaling spatial multiomics to cohort-scale research and clinical applications.