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Related Concept Videos

RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: May 5, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Shellac-Mediated Assembly of Nanoparticles for mRNA Delivery.

Meizhang Lu1, Jingqu Chen1, Zhixing Lin1

  • 1Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria, Australia.

Advanced Healthcare Materials
|April 21, 2026
PubMed
Summary
This summary is machine-generated.

Shellac stabilizes messenger RNA (mRNA) nanoparticles, overcoming the toxicity and efficiency tradeoff of cationic polymers. This novel platform enables effective mRNA delivery and expression in vivo via oral or intravenous routes.

Keywords:
biomedicinegene deliveryoral administrationself‐assemblysupramolecular chemistry

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Area of Science:

  • Biotechnology
  • Materials Science
  • Molecular Biology

Background:

  • Cationic polymers are widely researched for messenger RNA (mRNA) delivery, but face a tradeoff between high transfection efficiency and significant cytotoxicity.
  • High-molecular-weight polymers offer good transfection but cause toxicity, while low-molecular-weight polymers are less toxic but less effective.

Purpose of the Study:

  • To develop a versatile and safe mRNA delivery system by addressing the inherent toxicity-efficiency tradeoff of cationic polymers.
  • To utilize shellac (SL) as a stabilizing agent for mRNA complexed with low-molecular-weight cationic polymers.

Main Methods:

  • A shellac (SL)-mediated assembly strategy was employed to create mRNA-loaded nanoparticles.
  • Messenger RNA was complexed with low-molecular-weight cationic polymers and subsequently stabilized by SL under mild conditions.

Main Results:

  • The resulting SL-based nanoparticles demonstrated over 90% mRNA encapsulation efficiency.
  • These nanoparticles facilitated mRNA transfection across diverse cell types with negligible cytotoxicity.
  • The platform successfully enabled protein expression and gene editing in mice following both intravenous and oral administration.

Conclusions:

  • Shellac (SL) serves as a crucial component for directing the assembly and delivery of mRNA.
  • The SL-based nanoparticle platform offers a modular and versatile solution for nucleic acid delivery.
  • This approach holds significant potential for various biological applications requiring efficient and safe mRNA delivery.