Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Research Highlight: Ovine ESCs Bridge Naïve and Primed States.

Biology of reproduction·2026
Same author

Bovine Endometrium Drives and Responds to Divergence of In Vitro Produced Conceptus Biochemistry.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Impact of late embryonic and early fetal mortality on productivity of beef cows.

Translational animal science·2025
Same author

Human Recombinant Interleukin-6 and Leukemia Inhibitory Factor Improve Inner Cell Mass Cell Number but Lack Cryoprotective Activities on In Vitro-Produced Bovine Blastocysts.

Animals : an open access journal from MDPI·2025
Same author

An Overview of Reactive Oxygen Species Damage Occurring during In Vitro Bovine Oocyte and Embryo Development and the Efficacy of Antioxidant Use to Limit These Adverse Effects.

Animals : an open access journal from MDPI·2024
Same author

Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency.

Animals : an open access journal from MDPI·2024
Same journal

Reduced Circulating Polyamines in Polyendocrine Metabolic Ovarian Syndrome (PMOS) Patients and the Impact of Putrescine on Ovarian Function and Fertility in a Murine PMOS Model.

Biology of reproduction·2026
Same journal

DEVELOPMENTAL PROGRAMMING IN DOGS.

Biology of reproduction·2026
Same journal

Meiosis-Specific Cohesin in Mammalian Germ Cells.

Biology of reproduction·2026
Same journal

The Research of Lactylation in the Field of Reproduction.

Biology of reproduction·2026
Same journal

Mitochondria as Integrative Regulators of Ferroptosis in the Female Reproductive System.

Biology of reproduction·2026
Same journal

Retraction and replacement of: Navigation of Nanos germ cell specification factor to germ granules-posttranscriptional regulation hubs-across species.

Biology of reproduction·2026
See all related articles

Related Experiment Video

Updated: Apr 22, 2026

Author Spotlight: Advanced Enteroid Model for Studying Host-Pathogen Interactions
07:56

Author Spotlight: Advanced Enteroid Model for Studying Host-Pathogen Interactions

Published on: April 5, 2024

2.3K

Interleukin-6 and Leukemia Inhibitory Factor Differentially Modify Embryonic Disk Composition in Post Hatching Bovine

Mary A Oliver1, Vitor R G Mercadante1,2, Alan D Ealy1

  • 1School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.

Biology of Reproduction
|April 21, 2026
PubMed
Summary
This summary is machine-generated.

Interleukin-6 (IL6) supplementation after blastocyst formation in bovine embryos significantly enhances epiblast development to levels comparable to in utero development. Leukemia inhibitory factor (LIF) showed no significant effects.

Keywords:
blastocystbovineembryonic diskepiblasthypoblastinterleukin-6

More Related Videos

A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion
06:36

A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion

Published on: April 24, 2015

10.3K
A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
08:50

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

Published on: June 24, 2020

3.1K

Related Experiment Videos

Last Updated: Apr 22, 2026

Author Spotlight: Advanced Enteroid Model for Studying Host-Pathogen Interactions
07:56

Author Spotlight: Advanced Enteroid Model for Studying Host-Pathogen Interactions

Published on: April 5, 2024

2.3K
A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion
06:36

A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion

Published on: April 24, 2015

10.3K
A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
08:50

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

Published on: June 24, 2020

3.1K

Area of Science:

  • * Reproductive Biology
  • * Embryology
  • * Animal Science

Background:

  • * Proper embryonic disk formation is critical for successful pregnancy.
  • * Understanding factors influencing early embryonic development in vitro is essential for improving assisted reproductive technologies.
  • * Interleukin-6 (IL6) and Leukemia Inhibitory Factor (LIF) are cytokines with known roles in mammalian development.

Purpose of the Study:

  • * To investigate the effects of IL6 and LIF supplementation on bovine epiblast (EPI) and hypoblast (HYPO) development during extended in vitro culture.
  • * To determine the optimal timing for IL6 and LIF exposure to influence post-hatching embryonic development.

Main Methods:

  • * In vitro-produced (IVP) bovine embryos were cultured and supplemented with IL6 or LIF during specific developmental windows (days 5-12, 5-7, or 7-12 post-fertilization).
  • * Embryo survival and hatching rates were monitored.
  • * Immunofluorescent staining was used to assess EPI and HYPO cell numbers. Non-treated IVP embryos transferred in utero served as a reference.

Main Results:

  • * Neither IL6 nor LIF supplementation affected embryo survival or hatching rates.
  • * IL6 supplementation from days 5-12 or 7-12 post-fertilization significantly increased EPI cell numbers and decreased HYPO formation.
  • * IL6 supplementation from days 5-7 had no effect, indicating a requirement for IL6 post-blastocyst formation.

Conclusions:

  • * IL6 supplementation after blastocyst formation enhances bovine EPI development to levels comparable to in utero embryos.
  • * This enhancement of EPI development by IL6 may come at the cost of reduced HYPO formation.
  • * LIF did not replicate the effects of IL6 and had no significant impact on EPI or HYPO development.