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Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Novel Multienzymatic Cascade for the Conversion of Salicin From Plant Extract Into Salicylic Acid
Amandine Moreno1,2, Louis-Nicolas Gombault2, Adrien Cottias2
1Université de Reims Champagne-Ardenne, INRAE, FARE, UMR A 614, AFERE, Reims, France.
Abstract:
This study presents a novel multienzymatic strategy for the efficient and green production of valuable salicylic acid from plant-derived salicin. The biocatalytic route couples β-glucosidase for deglycosylation with laccase and xanthine oxidase (XO) for subsequent oxidation. The primary objective of this approach was to assess the feasibility of enzyme coupling within a single one-pot reaction. The results demonstrate that this strategy successfully enabled the transformation of salicylic alcohol into salicylic acid, mediated by a compatible laccase/TEMPO and XO system in a one-step process at pH 7 and 25°C. However, the integration of β-glucosidase into a one-pot system for direct salicin conversion was found to reduce the oxidative efficiency of XO, thus necessitating a sequential approach. Applying this optimized enzymatic cascade to a crude Salix purpurea bark extract initially encountered limitations in oxidation due to matrix complexity. Subsequent optimization of biocatalyst dosage delivered complete substrate oxidation and a 95% salicylic acid yield. This research significantly broadens the applicability of multienzymatic biocatalytic cascades, offering a promising and sustainable route for transforming natural compounds within complex plant extracts into high-value bioactive ingredients.
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