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Related Concept Videos

Interphase00:54

Interphase

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The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
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Interphase00:56

Interphase

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The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
Phases of Interphase
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Mitosis and Cytokinesis01:35

Mitosis and Cytokinesis

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In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
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Mitosis and Cytokinesis02:03

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In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
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Updated: Apr 22, 2026

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
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Orderly mitosis shapes interphase genome architecture.

Krishnendu Guin1, Adib Keikhosravi2, Raj Chari3

  • 1National Cancer Institute, NIH, Bethesda, United States.

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|April 21, 2026
PubMed
Summary
This summary is machine-generated.

Scientists used CRISPR screens to find key genes controlling 3D genome organization. They discovered that cell division processes significantly shape how centromeres are arranged in the cell nucleus during interphase.

Keywords:
CRISPR KO screencell biologycell cyclecell nucleuscentromeregenomehigh throughput imaginghuman

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Related Experiment Videos

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Area of Science:

  • Cell Biology
  • Genomics
  • Molecular Biology

Background:

  • The 3D genome architecture within the interphase nucleus is complex.
  • The molecular mechanisms governing global genome organization remain largely unknown.

Purpose of the Study:

  • To identify molecular mechanisms driving higher-order genome organization.
  • To understand the regulation of spatial genome architecture.

Main Methods:

  • Conducted high-throughput imaging-based CRISPR knockout screens targeting 1064 nuclear protein-coding genes.
  • Utilized centromere distribution at single-cell resolution as a surrogate marker for genome organization.
  • Employed multiple human cell lines for comprehensive analysis.

Main Results:

  • Identified major regulators of spatial centromere distribution, including nucleolar, kinetochore, cohesin, condensin, and nuclear pore complex components.
  • Demonstrated that centromere distribution changes require cell cycle progression.
  • Showed that depletion of specific mitotic factors alters centromere distribution in the subsequent interphase.

Conclusions:

  • Identified key molecular determinants of spatial centromere organization.
  • Established that ordered progression through mitosis is crucial for shaping interphase genome architecture.