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OrgDense: An automatic microfluidic centrifugal device for 3D cell condensation.

Jiawei Li^1,2,3, Yongde Cai^2,4, Ziqi Xiang^1,2,3

¹Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

The Review of Scientific Instruments

|April 21, 2026

Summary

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This summary is machine-generated.

We developed OrgDense, a novel method for high-throughput organoid development. This technique efficiently aggregates stem cells, overcoming bottlenecks in current organoid culture for accelerated timelines.

Area of Science:

Stem cell biology
Biotechnology
Regenerative medicine

Background:

Organoid development relies on embryoid body (EB) formation and gel-embedded differentiation.
Manual gel-embedding is a significant bottleneck, limiting the throughput of organoid generation.
Existing high-throughput EB formation methods are well-established.

Purpose of the Study:

To introduce OrgDense, a droplet-confined, high-throughput centrifugal cell condensation method.
To address the limitations of manual gel-embedding in organoid development.
To enhance paracrine signaling and accelerate organoid developmental timelines.

Main Methods:

OrgDense utilizes nanoliter volumes for droplet-confined cell aggregation.
Centrifugal force is employed to condense stem cells into compact pellets within a gel matrix.

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The method processes over 1000 organoid precursors per batch, reducing cell-occupied area by ~50%.

Main Results:

OrgDense achieved efficient stem cell aggregation within 1 hour in gel-free conditions, maintaining cell viability.
Condensed organoids developed reliably from single colonies with accelerated timelines.
The method demonstrated compatibility with multiple cell lines and primary cells.

Conclusions:

OrgDense significantly enhances organoid development throughput by overcoming manual gel-embedding bottlenecks.
The technique improves paracrine signaling through cell condensation, leading to faster organoid maturation.
OrgDense offers a robust and versatile platform for high-throughput organoid generation across various cell types.