Desulforhopalus glucosivorans sp. nov., a sulphate-reducing bacterium isolated from brackish lake sediment

A novel sulphate-reducing bacterium, strain 52FAK^T, was isolated from a sediment sample collected from a brackish lake, Lake Akkeshi, Japan. Growth occurred at 15-30 °C (optimum 22-25 °C), pH 6.9-8.2 (optimum 7.1-7.3) and 50-700 mM sodium chloride (optimum 150-300 mM). Strain 52FAK^T utilized hydrogen ga, various organic acids and alcohols as electron donors. Sulphate, sulphite, thiosulphate and nitrate served as electron acceptors. A distinctive feature is its ability to grow on glucose. The 52FAK^T genome consists of a circular chromosome with a length of 5.6 Mb and a G+C content of 43.6 mol%. The genome encodes a putative glucose-specific phosphotransferase system, which is likely involved in glucose uptake. Phylogenetic analyses placed strain 52FAKᵀ within the genus Desulforhopalus, with 16S rRNA sequence identities of 96.3% with Desulforhopalus singaporensis and 94.1% with Desulforhopalus vacuolatus. Based on phylogenetic and phenotypic properties, strain 52FAKᵀ is proposed as a new species, Desulforhopalus glucosivorans sp. nov., with the type strain 52FAKᵀ (=JCM 39400^T=DSM 113749^T).