Related Experiment Video
Updated: May 24, 2026
In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury
Published on: June 8, 2013
Lymph-interpositional flap transfer promotes lymphatic flow restoration: A rabbit hindlimb model supports the lymph
Jaemin Lee1, Su-Bin Lee1, Jae-Ho Chung1
1Department of Plastic and Reconstructive Surgery, Korea University Anam Hospital, Seoul, Republic of Korea.
Background:
Lymph-interpositional flap transfer (LIFT) is a novel lymphatic reconstruction technique that maintains alignment between donor and recipient lymphatic vessels-termed lymph axiality-without requiring lymph node harvest. Despite its conceptual rationale and growing clinical application, experimental validation remains limited. We aimed to evaluate the mechanistic impact of lymphatic interposition on lymphatic regeneration using a rabbit hindlimb model.
Methods:
A rabbit hindlimb model was developed. Limbs were assigned to either a LIFT (+) group (lymph axiality maintained via lymphatic interposition) or a LIFT (-) group (lymph axiality disrupted via rotational misalignment). After 6 weeks, outcome measures included limb volume assessment, indocyanine green (ICG) lymphography, histological analysis, and reverse transcription polymerase chain reaction (RT-PCR) analysis of lymphangiogenic and fibrotic markers.
Results:
The LIFT (+) group exhibited significantly reduced hindlimb volume at postoperative week 3 compared to the LIFT (-) group (p = 0.02), although no significant difference was observed at week 6. ICG lymphography revealed a significantly higher rate of lymphatic reconnection in the LIFT (+) group (p = 0.021). Histology confirmed the presence of lymphatics within the wound area and immunohistochemical staining to validate their lymphatic identity. RT-PCR demonstrated significantly elevated VEGF-C, CD31 and podoplanin expression (p < 0.05), along with reduced TGF-β levels in adjacent popliteal lymph nodes (p < 0.05).
Conclusions:
The LIFT procedure was associated with enhanced indicators of lymphatic restoration. Although preliminary, this first experimental evaluation of LIFT offers proof-of-concept evidence supporting lymphatic interposition as a key element of the lymph axiality concept.
