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Updated: Apr 23, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Published on: September 3, 2009
First multi-individual Neanderthal mitogenomes from north of the Carpathians
Andrea Picin1, Mateja Hajdinjak2, Wioletta Nowaczewska3
1Department of Chemistry G. Ciamician, University of Bologna, Via Selmi 2, 40126 Bologna, Italy.
Ancient Neanderthal teeth from Poland yielded eight new mitochondrial genomes, representing the oldest multi-individual Neanderthal genetic data in Central Europe. This finding highlights the region
Area of Science:
- Paleogenetics
- Paleoanthropology
- Archaeogenetics
Background:
- Neanderthal fossil and genetic records from Central-Eastern Europe are sparse.
- Stajnia Cave in Poland offers potential for new insights into European Neanderthal populations.
Purpose of the Study:
- To genetically characterize Neanderthal remains from Stajnia Cave.
- To establish the age and geographic distribution of Neanderthal mitochondrial DNA lineages in Central-Eastern Europe.
Main Methods:
- High-resolution study of nine Neanderthal teeth.
- Morphological assessment, radiocarbon dating, and complete mitochondrial genome sequencing.
- Molecular branch shortening for age estimation.
Main Results:
- Eight new Neanderthal mitochondrial genomes were recovered, representing at least seven individuals.
- The Stajnia Cave assemblage is dated to marine isotopic stage 5 (approximately 119,700-92,498 years ago).
- Identical mitochondrial DNA (mtDNA) found in three specimens suggests maternal relation.
Conclusions:
- This study presents the oldest multi-individual Neanderthal genetic assemblage from Central Europe.
- The identified mtDNA lineage was widespread across Europe before replacement by later Neanderthal types.
- Central-Eastern Europe is a key region for understanding Neanderthal geographic distribution and population dynamics.
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