Related Experiment Video
Updated: Apr 23, 2026

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
Published on: August 13, 2011
Distinct Mechanisms of Acetic Acid Inhibition in Thermophilic GH10 and GH11 Xylanases
Dan-Gyeong Han1, Beom Soo Kim1, In Jung Kim2
1Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Abstract:
Acetic acid generated during dilute acid or hydrothermal pretreatment of lignocellulosic biomass often acts as a potent inhibitor of glycoside hydrolases (GHs), including xylanases, thereby reducing the efficiency of xylan saccharification. This lowers xylose and xylooligosaccharide yields and overall carbon recovery in lignocellulosic biorefineries. In this study, we compared the inhibition patterns of a GH10 xylanase (TcrGH10) and a GH11 xylanase (TsaGH11) by acetic acid. Both enzymes exhibited only a modest loss of activity (~10-15%) between 0 and 0.8% acetic acid but were almost completely inactivated at 1.0%, suggesting a critical concentration range. Global fitting of kinetic data at 0, 0.3, and 0.75% acetic acid to inhibition models indicated mixed-type inhibition with a predominant non-competitive component for TcrGH10, whereas TsaGH11 predominantly followed an uncompetitive inhibition pattern. These findings elucidate family-specific inhibition behaviors and establish a critical acetic acid threshold, offering guidance for process optimization and the development of acetic acid-tolerant xylanases.
Related Concept Videos
Enzyme Inhibition
Indirect-Acting Cholinergic Agonists: Mechanism of Action
Reversible inhibitors like edrophonium bind to a specific part of the enzyme called the anionic catalytic site. They form noncovalent bonds, which means they are not strongly attached to the enzyme. This creates a temporary and less stable enzyme–inhibitor complex,...
Production of Organic Acids
Indirect-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
Reversible inhibitors display short to medium durations of action. Short-acting agents include simple alcohols with...
Acetals and Thioacetals as Protecting Groups for Aldehydes and Ketones
In the presence of multiple functional groups, when selective reduction of one group over the other is desired, groups like aldehydes and ketones that form acetals...
Diversity of Archaea IV

