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Related Concept Videos

Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

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In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
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Updated: Apr 23, 2026

Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
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Determination of Total Soluble Sugars in Pteridophytes Using the Anthrone Method.

Pablo Denova-Lozano1, Alejandra Chamorro-Flores2, Amparo B Cerón-Carpio3

  • 1Benemérita Universidad Autónoma de Puebla, Facultad de Ciencias Biológicas, México.

Current Protocols
|April 22, 2026
PubMed
Summary

This study details a method for quantifying total soluble sugars in pteridophytes using the anthrone assay. This protocol enables precise measurement from small leaf samples, aiding research into plant stress responses.

Keywords:
anthrone methodpteridophytessoluble sugarsstress tolerance

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Sugars are vital signaling molecules in plants, regulating physiological processes and stress responses.
  • Plants accumulate soluble sugars as osmolytes to protect against abiotic stress, stabilizing membranes and enzymes.
  • Accurate sugar quantification is key to understanding plant adaptation strategies.

Purpose of the Study:

  • To present a detailed protocol for extracting and quantifying total soluble sugars in pteridophytes.
  • To adapt the anthrone method for small leaf tissue samples (10 mg fresh weight).
  • To facilitate metabolic response studies in low-biomass plants and minimize population impact.

Main Methods:

  • Utilized the anthrone colorimetric assay for carbohydrate quantification.
  • Adapted the protocol for small sample volumes using ethanol extraction.
  • Incorporated spectrophotometry with absorbance measurement at 620 nm in 96-well plates.

Main Results:

  • Developed a reliable method for total soluble sugar extraction and quantification in pteridophytes.
  • The protocol is optimized for small sample sizes, making it suitable for rare or low-biomass species.
  • Enabled precise measurement of sugar content crucial for understanding plant stress physiology.

Conclusions:

  • The described protocol provides an efficient means to quantify total soluble sugars in pteridophytes.
  • This method supports research into plant biochemical and physiological strategies under various conditions.
  • Optimizing sample size is critical for studying plant metabolic responses and conserving plant populations.