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In silico development microsatellite marker panel and characterization for the endangered neotropical species Brycon
Daralyns B Macedo1,2,3, Carlos Willian D Dantas2,3, Sandy Ingrid A Alves2,3
1Federal Rural University of the Amazon, Applied Genetics Laboratory, Socio-environmental and Water Resources Institute, 2501, Tancredo Neves Ave., 66077-830 Belém, PA, Brazil.
Abstract:
This short communication presents novel molecular tools designed for the endangered species Brycon orbignyanus. In conservation studies, the development of population-specific markers tailored to particular geographic regions is essential. In this study, the prevalence of imperfect microsatellites highlights a evolution of this type of microsatellite in a population of Pelotas, Rio Grande do Sul. Therefore, the methodology used can serve as a guideline for genetic variability analysis even before conducting in vitro or in situ tests. These findings contribute significantly to technological prospecting and hold promise for applications in conservation programs. Additionally, they provide a basis for in vitro assays, such as PCR, to support species monitoring efforts.
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