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Updated: Apr 24, 2026
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
Prospective multicenter evaluation of the QuickNavi-Campylobacter assay in stool specimens
Shinji Hatakeyama1, Yumi Hirose2, Yusaku Akashi3
1Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan; Department of Infectious Diseases, Institute of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
Introduction:
A rapid diagnosis of Campylobacter infections is important for managing infectious gastroenteritis. Although stool culture is considered the gold standard, its sensitivity is limited, and it requires prolonged incubation times.
Methods:
We conducted a prospective multicenter study at nine healthcare facilities in Japan to evaluate a Campylobacter rapid antigen test using stool specimens between March 2024 and August 2025. Patients with suspected infectious gastroenteritis were consecutively enrolled and tested using QuickNavi-Campylobacter, and the results were compared with those obtained using the FilmArray Gastrointestinal Panel as the reference method. Discordant results were further evaluated using cultures and additional PCR assays.
Results:
A total of 410 patients were included in the final analysis. When the FilmArray Gastrointestinal Panel was used as the reference method, the positive concordance rate of QuickNavi-Campylobacter was 78.8%, and the negative and total concordance rates were 99.0% and 93.4%, respectively. The positive concordance rate decreased in specimens collected ≥6 days after the onset of symptoms (50.0%). Among the 29 patients who received antimicrobial agents before testing, Campylobacter spp. Were detected by the FilmArray GI Panel in five patients, whereas only one of these patients was positive by the antigen assay.
Conclusions:
QuickNavi-Campylobacter showed acceptable concordance with the FilmArray Gastrointestinal Panel in a real-world multicenter setting, and its diagnostic utility may be more evident in the early phase of illnesses, although prior antimicrobial exposure may reduce its sensitivity.
