Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Analysis of Population Pharmacokinetic Data01:12

Analysis of Population Pharmacokinetic Data

980
Analysis of population pharmacokinetic data involves studying the behavior of drugs within diverse populations to understand their pharmacokinetic parameters. Traditional pharmacokinetic methods typically involve collecting samples from a few individuals and estimating these parameters. While these methods are commonly used, they have limitations in capturing the variability in drug response among individuals or heterogeneous populations. Population pharmacokinetics is employed to address these...
980
Summation Notation01:25

Summation Notation

393
Sigma notation, also known as summation notation, provides a concise method for representing the sum of a sequence of terms that follow a regular pattern. It utilizes the uppercase Greek letter sigma (∑), A typical expression is:In this form, k the index of summation is 1, the starting value, and n the ending value. The term ak​ represents the general term of the sequence.For example, the increasing sequence 5, 7, 9, ..., 23 over 10 terms can be expressed as:This simplifies the...
393

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The seminal proteome of a monandrous fly, Drosophila subobscura.

Proceedings. Biological sciences·2026
Same author

The comet assay as a tool for human biomonitoring of exposure to environmental and occupational agents - A summary of systematic reviews and meta-analyses.

Mutation research. Reviews in mutation research·2026
Same author

Hepatic cellular stress response pathways exhibit species differences in basal and inducible activity.

Toxicological sciences : an official journal of the Society of Toxicology·2026
Same author

An AI-Ready Phosphorylation Meta-Analysis for <i>Saccharomyces cerevisiae</i>.

Journal of proteome research·2026
Same author

The promise of AlphaFold for gene structure annotation.

Nucleic acids research·2026
Same author

Rare variants in embryonic development and cell signalling genes in syndromic and non-syndromic orofacial clefts: evidence from a Colombian Caribbean cohort.

Journal of human genetics·2026

Related Experiment Video

Updated: Apr 24, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

8.8K

A Landscape Analysis of Human SUMOylation.

Shireen S Al-Momani1, Kerry Rambsbottom1, Andrew Collins1

  • 1Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.

Molecular & Cellular Proteomics : MCP
|April 22, 2026
PubMed
Summary

This study presents the Human SUMO Build, a comprehensive map of human SUMOylation sites identified using mass spectrometry. This resource reveals key features of SUMOylation and its links to disease and other protein modifications.

Keywords:
SUMOylationcomputational proteomicsfalse localization ratehuman SUMO proteomemass spectrometry

More Related Videos

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

6.6K
SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.5K

Related Experiment Videos

Last Updated: Apr 24, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

8.8K
In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

6.6K
SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.5K

Area of Science:

  • Biochemistry
  • Proteomics
  • Molecular Biology

Background:

  • SUMOylation, a post-translational modification (PTM), plays crucial roles in physiological and pathological processes but remains understudied.
  • Advances in mass spectrometry (MS) and enrichment strategies enable large-scale mapping of SUMOylation sites.

Purpose of the Study:

  • To construct a comprehensive, high-confidence reference set of human SUMOylation sites by reanalyzing publicly available MS-based datasets.
  • To characterize the features of SUMOylation sites, including their location, associated motifs, and relationship with other PTMs and disease variants.

Main Methods:

  • Reanalysis of public MS-based SUMOylation datasets.
  • Integration of database searching (Trans-Proteomic Pipeline) and statistical analysis for false localization rate (FLR) estimation.
  • Classification of SUMOylated lysine sites into confidence tiers and comparison with non-SUMOylated lysines.

Main Results:

  • The Human SUMO Build contains 35,721 SUMOylation sites across 6,146 proteins with <5% FLR.
  • SUMOylated lysines are enriched in intrinsically disordered regions and near phosphosites, and associated with disease variants.
  • Identified canonical, inverted, and novel SUMOylation motifs and found overlap with ubiquitination sites, particularly at disease-associated positions.

Conclusions:

  • The Human SUMO Build provides a valuable resource for understanding SUMOylation.
  • SUMOylation site characteristics and co-occurrence with ubiquitination offer insights into regulatory mechanisms and disease associations.
  • The data is publicly available to facilitate further research and predictive modeling.