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Updated: Apr 24, 2026
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
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The long non-coding RNA landscape of endurance exercise training
Bernardo Bonilauri1, Gregory R Smith2, Archana N Raja3
1Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA; Department of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Molecular Metabolism
|April 22, 2026
Summary
Physical exercise significantly alters long non-coding RNA (lncRNA) expression across various rat tissues, with notable changes in adipose tissue and the hypothalamus. This study reveals exercise-responsive lncRNAs, offering insights into exercise as medicine.
Area of Science:
- Molecular Biology
- Genomics
- Exercise Physiology
Background:
- Long non-coding RNAs (lncRNAs) are crucial regulators of cellular functions.
- The role of lncRNAs in response to physical activity and exercise training is not well understood.
Purpose of the Study:
- To comprehensively analyze lncRNA expression patterns in multiple tissues following endurance training.
- To characterize the tissue-specific responses of lncRNAs to exercise.
Main Methods:
- Analysis of lncRNA expression in 18 rat tissues after an 8-week progressive endurance training program.
- Comparison of lncRNA characteristics (abundance, length, GC content) with protein-coding genes.
- Investigation of correlations between lncRNA expression and immune-related coding genes.
Main Results:
- lncRNA expression patterns were largely tissue-specific, with 759 unique differentially expressed lncRNAs identified.
- Most pronounced changes occurred in adipose tissues, hypothalamus, and adrenal gland.
- Significant sex differences in lncRNA regulation were observed in response to exercise training.
Conclusions:
- Exercise training induces significant, tissue-specific changes in lncRNA expression.
- lncRNAs may play a role in mediating the immune response to exercise, particularly in adipose tissue.
- This research provides a foundation for understanding exercise's molecular mechanisms and developing lncRNA-based therapeutics.
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