Hsa_circ_0002111 implicates KTC-1 cell motility and modulates migration and invasion via miR-432-5p/CDKN2B axis

Area of Science:

• Molecular Biology
• Oncology
• Gene Regulation

Background:

• Papillary thyroid carcinoma (PTC) is a prevalent endocrine malignancy.
• Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer progression.
• Hsa_circ_0002111 is highly expressed in PTC, indicating its potential involvement in tumorigenesis.

Purpose of the Study:

• To investigate the role and mechanism of hsa_circ_0002111 in promoting PTC progression.
• To elucidate the circRNA/miRNA/mRNA regulatory axis involving hsa_circ_0002111.
• To assess the potential of hsa_circ_0002111 as a therapeutic target in PTC.

Main Methods:

• Quantitative reverse transcription-PCR (Q-RT-PCR) for gene expression analysis.
• Dual-luciferase reporter assays, AGO2-RIP to confirm molecular interactions.
• Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell assays to evaluate cellular phenotypes.

Main Results:

• Silencing hsa_circ_0002111 significantly inhibited viability, migration, and invasion of KTC-1 cells.
• Hsa_circ_0002111 functions as a molecular sponge for miR-432-5p, suppressing its activity.
• miR-432-5p targets the 3' untranslated region (3'UTR) of CDKN2B, inhibiting its expression; CDKN2B knockdown phenocopied hsa_circ_0002111 silencing effects.

Conclusions:

• The hsa_circ_0002111/miR-432-5p/CDKN2B axis plays a crucial role in papillary thyroid carcinoma progression.
• Hsa_circ_0002111 acts as a novel regulator in PTC, impacting cell viability, migration, and invasion.
• Hsa_circ_0002111 represents a potential therapeutic target for thyroid cancer, offering new clinical research directions.