Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.4K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.4K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.0K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.0K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.0K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.0K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.0K
Histone Modification02:32

Histone Modification

14.4K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The relationship between family socioeconomic status and cultural background on the career self-determination of Chinese and Kazakhstani students.

Frontiers in psychology·2026
Same author

Dual resistance to asparaginase and PD-1 blockade in extranodal natural killer/T-cell lymphoma: dismal outcomes from a multicenter cohort.

Haematologica·2026
Same author

Clinical significance of high-density lipoprotein cholesterol and its dynamic change in patients with lymphoma-associated hemophagocytic lymphohistiocytosis.

Therapeutic advances in medical oncology·2026
Same author

Neutrophil extracellular traps in thrombosis of hematologic malignancies: Underlying mechanisms and therapeutic opportunities (Review).

International journal of molecular medicine·2026
Same author

Heterogeneous and nonlinear associations between depressive symptoms and functional disability in older Chinese adults.

BMC psychology·2026
Same author

Mume Fructus Total Flavonoids Modulate miR-145-3p Expression to Inhibit Lipopolysaccharide-induced Inflammatory Cytokine Production in BV2 Cells.

Combinatorial chemistry & high throughput screening·2026

Related Experiment Video

Updated: Apr 24, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

6.8K

The reprogramming and function of H4K20me1 during early embryo development.

Xiangrui Meng1,2, Zhen He3,4, Dong Fang5

  • 1The First Affiliated Hospital of Zhengzhou University & Institute of Reproductive Health, Henan Academy of Innovations in Medical Science, Zhengzhou, China.

EMBO Reports
|April 22, 2026
PubMed
Summary

Histone H4 lysine 20 mono-methylation (H4K20me1) is vital for embryonic development, regulating gene activation and chromatin accessibility. Its dynamic reprogramming after fertilization is essential for early embryogenesis and genome stability.

More Related Videos

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples
12:47

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

15.4K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

6.7K

Related Experiment Videos

Last Updated: Apr 24, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

6.8K
Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples
12:47

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

15.4K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

6.7K

Area of Science:

  • Epigenetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Histone modifications regulate chromatin dynamics and embryonic development.
  • Histone H4 lysine 20 mono-methylation (H4K20me1) is an essential epigenetic mark linked to gene expression and genome stability.
  • The reprogramming and functional roles of H4K20me1 in early embryogenesis are not well understood.

Purpose of the Study:

  • To map the genome-wide distribution of H4K20me1 in early embryos of mouse, human, and zebrafish.
  • To investigate the functional necessity of H4K20me1 and its methyltransferase SET8 during embryogenesis.
  • To elucidate the mechanistic role of H4K20me1 in zygotic genome activation (ZGA) and chromatin accessibility.

Main Methods:

  • Genome-wide mapping of H4K20me1 distribution in mouse, human, and zebrafish early embryos.
  • Functional perturbation studies involving SET8, the H4K20me1 methyltransferase.
  • Analysis of H4K20me1's role in regulating RNA synthesis, transcription, and chromatin accessibility during ZGA.

Main Results:

  • H4K20me1 shows broad genome-wide distribution with species-specific features in early embryos.
  • H4K20me1 is enriched in gene bodies and dynamically reprogrammed post-fertilization.
  • SET8 perturbation leads to developmental arrest, confirming H4K20me1's essential role.
  • H4K20me1 is critical for ZGA, regulating transcription and chromatin accessibility.

Conclusions:

  • H4K20me1 undergoes dynamic reprogramming essential for early embryonic development.
  • H4K20me1 plays a crucial role in zygotic genome activation, transcription, and chromatin accessibility.
  • These findings offer insights into the epigenetic regulation of early embryogenesis.