Related Experiment Video
Updated: Apr 24, 2026
07:50
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
7.0K
RASSF2 promoter hypermethylation determines malignant and microenvironmental features in lung cancer
Yingying Han1, Wenying Jiang1, Qingning Chen1
1Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Genes and Immunity
|April 22, 2026
Summary
Ras-association domain family 2 (RASSF2) acts as a tumor suppressor in lung adenocarcinoma (LUAD). RASSF2 inhibits LUAD cell proliferation and metastasis, and its methylation correlates with poor patient survival and altered immune signaling.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Lung adenocarcinoma (LUAD) presents a complex microenvironment challenging clinical management.
- The Ras-association domain family (RASSF) was analyzed, identifying RASSF2 as a potential tumor suppressor in LUAD.
Purpose of the Study:
- To investigate the clinical significance and functional mechanisms of RASSF2 in LUAD.
- To explore RASSF2's role in LUAD cell proliferation, migration, and invasion.
- To elucidate the molecular mechanisms underlying RASSF2 methylation in LUAD.
Main Methods:
- Cell proliferation was assessed using CCK-8, EdU, and colony formation assays.
- Cell migration and invasion were evaluated via Transwell and wound healing assays.
- RNA sequencing was performed on LUAD tissue samples to analyze RASSF2 methylation mechanisms.
Main Results:
- RASSF2 overexpression significantly inhibited LUAD cell proliferation, migration, and invasion.
- RASSF2 promoter hypermethylation was associated with significantly shorter overall survival in LUAD patients.
- RNA-seq revealed RASSF2 methylation correlates with NF-κB pathway activation and T-cell dysregulation.
Conclusions:
- RASSF2 demonstrates a tumor-suppressive role in LUAD.
- RASSF2 methylation is a prognostic biomarker for LUAD.
- RASSF2 presents potential as an immunotherapeutic target for LUAD treatment.
Related Concept Videos
02:17The Tumor Microenvironment
6.3K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.3K
01:37Epigenetic Regulation
3.3K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.3K
01:46Epigenetic Regulation
28.6K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
28.6K
02:23Abnormal Proliferation
4.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
02:38The Ras Gene
5.7K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
5.7K
03:03mTOR Signaling and Cancer Progression
3.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.6K