Intravitreal Injections in Mouse Models of Eye Disease

Intravitreal injections are a commonly used technique in the treatment of some vitreoretinal diseases. In clinical situations, intravitreal injections are often used to deliver drugs that treat diseases such as age-related macular degeneration, retinal vein occlusion, and uveitis. Many preclinical studies in animals such as mice utilize intravitreal injections to test neuroprotective treatments and gene therapies before clinical use can be indicated. Herein, we describe our method and favorable practices for intravitreal injection into the eye of both preweaned and adult mice.