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Andrographolide Modulates Fibrogenic and Oxidative Stress Responses in Human Lung Fibroblasts
Yu-Hsin Tseng1, Yen-Hsien Wu1, Yi-Ching Liu1
1Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
The Kaohsiung Journal of Medical Sciences
|April 23, 2026
Summary
Andrographolide (ANDRO) combats pulmonary fibrosis by restoring glutathione (GSH) metabolism and reducing oxidative stress in lung fibroblasts. This study reveals GSH metabolism as a key antifibrotic mechanism for ANDRO.
Area of Science:
- Pulmonology
- Cell Biology
- Pharmacology
Background:
- Pulmonary fibrosis involves fibroblast activation and extracellular matrix buildup.
- Andrographolide (ANDRO) shows potential antifibrotic effects, but mechanisms are unclear.
Purpose of the Study:
- Investigate ANDRO's effects on transforming growth factor-beta 1 (TGF-β1)-induced fibrogenesis.
- Elucidate the molecular mechanisms of ANDRO's antifibrotic action.
Main Methods:
- Used transcriptomic analysis to study gene expression changes.
- Assessed glutathione (GSH) levels and reactive oxygen species (ROS) accumulation in human lung fibroblasts.
Main Results:
- ANDRO reversed TGF-β1-altered genes related to fibrogenesis and GSH metabolism.
- ANDRO restored GSH levels suppressed by TGF-β1.
- ANDRO inhibited TGF-β1-induced ROS accumulation.
Conclusions:
- Glutathione (GSH) metabolism is a novel mechanism for ANDRO's antifibrotic effects.
- ANDRO demonstrates potential as a therapeutic agent for pulmonary fibrosis by targeting GSH metabolism and oxidative stress.