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LINC01128 Affects Triple-Negative Breast Cancer Progression Through Targeting miR-32-5p

Min Xiong1, Quanjun Yang1, Le Cheng1

  • 1Department of Oncology, Affiliated Renhe Hospital of China Three Gorges University, Yichang, Hubei, 443000, People's Republic of China.

Breast Cancer (Dove Medical Press)
|April 23, 2026
PubMed
Abstract

Insights

Long non-coding RNA LINC01128 is highly expressed in triple-negative breast cancer (TNBC) and promotes tumor progression by suppressing miR-32-5p. This suggests LINC01128 is a potential therapeutic target for TNBC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) is an aggressive subtype with limited therapeutic options.
  • Long non-coding RNAs (lncRNAs) play crucial roles in cancer development and progression.
  • Understanding the molecular mechanisms underlying TNBC is essential for developing novel treatments.

Purpose of the Study:

  • To investigate the expression and clinical significance of LINC01128 in TNBC.
  • To determine if LINC01128 regulates TNBC cell behaviors by targeting miR-32-5p through the competing endogenous RNA (ceRNA) mechanism.
  • To explore LINC01128 as a potential therapeutic target for TNBC.

Main Methods:

  • Quantitative real-time PCR (qPCR) to measure LINC01128 and miR-32-5p expression in TNBC tissues and cell lines.
  • Dual-luciferase reporter assay to confirm the direct binding between LINC01128 and miR-32-5p.
  • Cell proliferation, apoptosis, and migration assays (CCK-8, flow cytometry, Transwell) to assess the functional impact of LINC01128.
  • Bioinformatic analyses (miRDB, miRWalk) for target gene prediction and pathway enrichment.

Main Results:

  • LINC01128 was significantly upregulated in TNBC tissues and cells compared to normal controls.
  • High LINC01128 expression correlated with advanced TNBC and served as an independent risk factor.
  • miR-32-5p was downregulated in TNBC and showed a negative correlation with LINC01128.
  • LINC01128 promoted TNBC cell proliferation and migration while inhibiting apoptosis by sponging miR-32-5p.
  • Target genes of miR-32-5p were enriched in cancer-related pathways.

Conclusions:

  • LINC01128 is highly expressed in TNBC and drives tumor progression.
  • The LINC01128/miR-32-5p ceRNA axis plays a critical role in regulating TNBC cell behaviors.
  • LINC01128 represents a promising molecular marker and therapeutic target for TNBC.
Keywords:
LINC01128apoptosismiR-32-5pmigrationproliferationtriple-negative breast cancer

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