Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultrafast Plasmonic Nanocavity Thermocycler for Direct Real-Time Reverse Transcription PCR at a Point-of-Care Level.

ACS applied materials & interfaces·2026

Same author

A Pollen-Enhanced Bionic Mechanoreceptor Induced by Asymmetric Ionic Convection in Hydrogel for Sensory-Augmented Prostheses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Optimization of polyethylene glycol-based isolation of exosomes from mesenchymal stem cells for regenerative medicine applications.

Stem cell research & therapy·2026

Same author

Aberrant TIMM8B alternative splicing compromises mitochondrial respiratory chain integrity and redox homeostasis.

Free radical biology & medicine·2026

Same author

Bronopol and benzisothiazolinone exhibit synergistic toxicity with enhanced cell stress responses exceeding the impacts of individual compounds.

Environmental toxicology and pharmacology·2026

Same author

Delayed anterior mitral leaflet perforation after transcatheter aortic valve implantation with prosthesis-leaflet interaction.

European heart journal. Cardiovascular Imaging·2026

Search research articles

Related Experiment Video

Updated: Apr 24, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

MASQ: A multiplex qPCR platform for quantitative alternative splicing analysis in clinical specimens.

Hyejeong Jeong¹, Namjoon Cho¹, Jong-Il Park²

¹Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 34134, Republic of Korea.

Molecular Therapy. Nucleic Acids

|April 23, 2026

Summary

Multiplex alternative splicing quantification (MASQ) precisely measures splicing events using a novel qPCR platform. This method enhances precision and sensitivity for alternative splicing analysis, aiding disease mechanism studies and precision medicine.

Keywords:

Diagnostics and Biosensors HTRA2 MASQ MT Oligonucleotides PBRM1 alternative splicing biomarker multiplex qPCR precision medicine therapeutic monitoring

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Related Experiment Videos

Last Updated: Apr 24, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Alternative splicing quantification is crucial for understanding diseases and developing precision medicine.
Conventional methods lack resolution and precision for similar-sized exons.

Purpose of the Study:

To introduce multiplex alternative splicing quantification (MASQ), a dual TaqMan probe qPCR platform.
To enable precise quantification of alternative splicing events with improved accuracy and sensitivity.

Main Methods:

MASQ utilizes FAM-labeled probes for alternative exons and HEX-labeled probes for constitutive exon normalization.
A model system using PBRM1 exon 27 was employed to demonstrate analytical performance.
CRISPR-engineered cells validated probe specificity.

Main Results:

MASQ demonstrated high linearity (R ² > 0.98), precision (intra-assay CV < 9%), and sensitivity (LOD 195 fg).
Significantly elevated PBRM1 exon 27 inclusion was observed in uterine corpus endometrial carcinoma tissues.
The platform was successfully adapted for HTRA2 exon 7 quantification and splice-switching oligonucleotide monitoring.

Conclusions:

MASQ is a broadly applicable platform for precise alternative splicing analysis.
This technology supports biomarker discovery, diagnostic development, and therapeutic monitoring.