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Updated: Apr 25, 2026
A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles
Published on: April 10, 2019
Protocol for spectrophotometric determination of titanium microparticles in biological samples via Ti(IV)-H2O2
Li Xie1, Danyal A Siddiqui2, Georgios A Kotsakis2
1Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ 07974, USA; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China, Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
Abstract:
Titanium (Ti) particles implicated in peri-implant inflammation require reliable quantification in biological samples. Here, we present a spectrophotometric protocol for the analysis of Ti particles. We describe steps for implementing optimized pretreatment to remove organic matrices and performing hot-acid chemical digestion. We then detail procedures for quantifying Ti concentration via a Ti(IV)-hydrogen peroxide (H2O2) chromogenic reaction and validating detection accuracy. This protocol provides an accessible, scalable technique for quantifying Ti particles in laboratories with limited access to advanced analytical instrumentation.
