Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.1K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

10.1K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
10.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.0K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.0K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Omics landscapes of hepatic echinococcosis: bulk foundations, emerging single-cell studies, and analytical considerations.

Frontiers in immunology·2026
Same author

IL1R2 identified as a key hub gene regulating vascular endothelial function in Kawasaki disease.

Scientific reports·2026
Same author

Reduced myocardial NRG-1/ErbB4 signaling is associated with STING-linked macrophage pyroptotic signaling in sepsis-induced cardiac injury.

International immunopharmacology·2026
Same author

Taurine supplementation in fish meal-free diet improved growth, alleviated hypoxic-induced gill injury associating with Ca<sup>2+</sup> homeostasis and endoplasmic reticulum stress in sub-adult grass carp (<i>Ctenopharyngodon idella</i>).

Animal nutrition (Zhongguo xu mu shou yi xue hui)·2026
Same author

Dimerization-induced conformational transitions of yeast iso-1 cytochrome <i>c</i>.

Magnetic resonance letters·2026
Same author

Functional characterization of TONSOKU in Cyathula officinalis reveals its role in DNA damage and root development.

BMC plant biology·2026

Related Experiment Video

Updated: Apr 25, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

7.8K

Dynamics of the SS Loop Regulates SARM1's Catalysis.

Jian Yuan Yang1, Wei Ming He2, Wan Hua Li1,2

  • 1School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China.

ACS Chemical Neuroscience
|April 23, 2026
PubMed
Summary

Researchers discovered that the SARM1-specific loop

Keywords:
ADP-ribosyl cyclasePAOPC6SARM1SS loopcADPRcysteinezinc ion

More Related Videos

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

9.7K
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
11:27

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

8.2K

Related Experiment Videos

Last Updated: Apr 25, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

7.8K
Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

9.7K
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
11:27

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

8.2K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Axonal degeneration (AxD) is a hallmark of neurodegenerative diseases.
  • SARM1 is a critical pro-degenerative enzyme (NADase) activated by conformational changes.

Purpose of the Study:

  • To identify the regulatory mechanism of SARM1 activation.
  • To elucidate the role of the SARM1-specific (SS) loop in SARM1's catalytic activity.

Main Methods:

  • Site-directed mutagenesis
  • Enzyme activity assays
  • Molecular dynamics simulations
  • Biochemical inhibition studies

Main Results:

  • Dynamic conformational transitions of the SS loop, including His640 flipping, are crucial for SARM1 NAD recruitment and catalysis.
  • Zinc ions inhibit SARM1 by binding to a C2H2 motif in the SS loop, restricting His640 movement.
  • Chemical tethering of SS loop cysteines also inhibits SARM1 activity by limiting conformational dynamics.

Conclusions:

  • SS loop dynamics are essential for SARM1 NADase activity.
  • This finding provides mechanistic insight into SARM1 regulation.
  • The study lays the groundwork for developing SARM1-targeted therapeutics for neurodegenerative disorders.