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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

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Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Updated: Apr 25, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Chromatography-Free Analysis of Mixtures Using a Two-Dimensional Mass Spectrometry (2DMS)-Enabled Quadrupole

Steven Wright1, Nathan Cassidy1, Alex Colburn1,2

  • 1Verdel Instruments Ltd, 154k Brook Drive, Milton Park, Abingdon, Oxfordshire OX14 4SD, U.K.

Analytical Chemistry
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PubMed
Summary

This study demonstrates two-dimensional mass spectrometry (2DMS) on a quadrupole time-of-flight instrument, enabling high-resolution analysis without chromatography. This advancement makes advanced 2DMS accessible for peptide mixture sequencing.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Proteomics

Background:

  • Two-dimensional mass spectrometry (2DMS) is a data-independent acquisition technique for analyzing complex mixtures.
  • High-resolution 2DMS has been limited to Fourier Transform Ion Cyclotron Resonance (FTICR) instruments, which are not widely accessible.
  • There is a need for high-resolution 2DMS methods compatible with more common instrumentation.

Purpose of the Study:

  • To demonstrate the feasibility of high-resolution 2DMS using a quadrupole time-of-flight (QToF) mass spectrometer.
  • To introduce and validate the application of stored waveform ion radius modulation (SWIM) for 2DMS on a QToF instrument.
  • To enable simultaneous sequencing of individual components within peptide mixtures.

Main Methods:

  • Implementation of stored waveform ion radius modulation (SWIM) on ions trapped in a quadrupole.
  • Utilizing broadband dipolar excitation and radius-dependent fragmentation for ion modulation.
  • Analysis of modulated ions by a time-of-flight (ToF) mass analyzer.
  • Employing collision-induced dissociation (CID) and/or ultraviolet photodissociation (UVPD) for fragmentation.

Main Results:

  • Successful experimental demonstration of 2DMS on a QToF mass spectrometer.
  • Generation of high-resolution 2D mass spectra with accurate mass assignments.
  • Individual and simultaneous sequencing of peptides within a mixture was achieved.
  • SWIM effectively modulated precursor and product ion signals for 2D analysis.

Conclusions:

  • High-resolution 2DMS is achievable on widely available QToF instruments.
  • SWIM is a key enabling technology for QToF-based 2DMS.
  • This method significantly expands the accessibility of advanced 2DMS for peptide mixture analysis and sequencing.