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Related Experiment Video

Updated: Apr 25, 2026

Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication
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OCTAVie: Open-source Capture Technology for Animal Vocalization in ultrasonic range.

Laura Durieux1, Jean-Baptiste Kammerer1, Simon Schmitt2

  • 1Université de Strasbourg, Centre National de La Recherche Scientifique, ICube Laboratory, UMR 7357, Strasbourg, France.

Hardwarex
|April 24, 2026
PubMed
Summary

Researchers developed an affordable, open-hardware system for real-time rodent ultrasonic vocalization (USV) analysis. This tool simplifies data processing and reduces storage needs for neuroscience research.

Keywords:
FPGAMicrophoneNeuroscienceOpen-hardwareReal-time sonogramUltrasonic vocalizations

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Area of Science:

  • Neuroscience
  • Bioacoustics
  • Hardware Engineering

Background:

  • Rodent ultrasonic vocalizations (USVs) are crucial for studying emotion, social behavior, and disease models.
  • Existing commercial recording systems are costly and produce large datasets requiring extensive post-processing.
  • There is a need for accessible, efficient tools for USV analysis in neuroscience.

Purpose of the Study:

  • To present an open-hardware acquisition system for real-time ultrasonic vocalization (USV) sonogram computation and display.
  • To reduce data storage requirements and analysis workload for rodent USV research.
  • To provide a low-cost, modular, and reproducible platform for studying ultrasonic communication.

Main Methods:

  • Designed an integrated acquisition chain: custom ultrasonic microphone (up to 100 kHz), analog front-end with filtering, FPGA for real-time FFT and power calculation, and a lightweight software interface.
  • Openly released all design files (PCB layouts, VHDL, C software) to ensure reproducibility.
  • Validated the system with synthetic signals and experimental recordings.

Main Results:

  • The system computes and displays sonograms in near real-time, significantly reducing storage needs.
  • Achieved over 50% reduction in raw data storage compared to traditional microcontroller-based systems.
  • Maintained high frequency (∼0.39 kHz) and temporal (∼2.5 ms) resolutions, sufficient for analyzing 22 kHz and 50 kHz USVs.

Conclusions:

  • The developed open-hardware platform offers a low-cost, modular, and transparent solution for rodent USV analysis.
  • This system lowers the barrier to entry for neuroscientists studying ultrasonic communication.
  • Facilitates efficient data handling and analysis, accelerating research in emotion, social behavior, and disease models.