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Development of a qualitative detection method for the pSIM1278 construct in genetically modified potato using

Miyu Sugino1, Keisuke Soga1, Jumpei Narushima1

  • 1Division of Biochemistry, National Institute of Health Sciences, Kawasaki-shi, Japan.

GM Crops & Food
|April 24, 2026
PubMed
Summary

A new real-time PCR method can detect all Innate® genetically modified (GM) potato events simultaneously. This tool aids in monitoring GM potatoes and ensuring informed consumer choice regarding food products containing the pSIM1278 genetic construct.

Keywords:
AcrylamideGM potatodetection methodlate blightpSIM1278real-time PCR

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Area of Science:

  • Agricultural Biotechnology
  • Food Science
  • Molecular Biology

Background:

  • Innate® genetically modified (GM) potatoes offer traits like black spot resistance and reduced acrylamide.
  • First-generation (E12) and second-generation (X17, Y9, Z6) Innate® events are approved for food use globally.
  • Monitoring these GM potatoes is crucial for labeling and consumer choice.

Purpose of the Study:

  • To develop a method for simultaneous detection of all Innate® GM potato events.
  • To target the shared pSIM1278 genetic construct for broad detection.
  • To provide a tool for monitoring GM potatoes in the food supply chain.

Main Methods:

  • Development of a real-time PCR assay targeting the pSIM1278 genetic construct.
  • Evaluation of the assay's sensitivity and specificity.
  • Application of the assay to survey imported potato products in Japan.

Main Results:

  • A single real-time PCR reaction can screen for all Innate® GM potato events.
  • The developed assay demonstrated high sensitivity and specificity.
  • The method was practically applied to detect GM potatoes in imported food products.

Conclusions:

  • A novel real-time PCR method enables simultaneous detection of Innate® GM potato events.
  • This assay is a valuable tool for food monitoring and regulatory compliance.
  • Ensures transparency and informed consumer choice regarding GM potato products.