Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cnidarian-algal partnerships structure bacterial communities during strobilation in <i>Cassiopea xamachana</i>.

ISME communications·2026

Same author

Metabolic Remodeling of the Parkinson's Disease Frontal Cortex Revealed by LC-MS/MS Metabolomics.

Biomolecules·2026

Same author

High-resolution LC-MS/MS analysis of brain N-glycans reveals composition-specific changes in Parkinson's disease.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026

Same author

Qualitative Characterization of Neu5Gc-Containing N-Glycans in Commercial Cheddar Cheese Products Using Nano-UHPLC-ESI-MS/MS-A Pilot Study.

International journal of molecular sciences·2026

Same author

Parkinson's Disease-Associated Remodeling of Synaptic <i>N</i>-Glycans in the Human Prefrontal Cortex.

Journal of proteome research·2026

Same author

Role of Endocannabinoid System Perturbation in Organophosphate-Mediated Metabolic Impairment and Neuroinflammation.

Basic & clinical pharmacology & toxicology·2026

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Direct Comparison of MRM-MS and PRM-MS Methods for Quantitative Ganglioside Analysis.

Akeem Sanni¹, Abderrahmane Koraich¹, Judith Nwaiwu¹

¹Chemistry and Biochemistry Department, Texas Tech University, Lubbock, Texas 79409, United States.

Journal of the American Society for Mass Spectrometry

|April 24, 2026

Summary

Parallel reaction monitoring (PRM) offers superior ganglioside analysis compared to multiple reaction monitoring (MRM). PRM provides enhanced sensitivity, structural detail, and isomer specificity for complex biological samples.

Keywords:

CID HCD LC-MS/MS MRM PRM gangliosides

More Related Videos

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Published on: March 23, 2017

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Related Experiment Videos

Last Updated: Apr 25, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Published on: March 23, 2017

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Area of Science:

Biochemistry
Analytical Chemistry
Neuroscience

Background:

Gangliosides are crucial glycosphingolipids in neuronal signaling and cancer.
Quantitative ganglioside analysis is challenging due to low abundance, heterogeneity, and isomeric complexity.
Targeted mass spectrometry (MS) methods like MRM and PRM are vital for ganglioside quantification.

Purpose of the Study:

To systematically compare Parallel Reaction Monitoring (PRM) and Multiple Reaction Monitoring (MRM) for native ganglioside quantification.
To optimize a targeted LC-MS/MS workflow for ganglioside analysis.
To evaluate the sensitivity, specificity, and structural information provided by PRM versus MRM.

Main Methods:

Developed and optimized a targeted LC-MS/MS workflow.
Compared PRM on a Q-Exactive HF Orbitrap with MRM on a TSQ Vantage triple quadrupole.
Optimized collision energies (NCE/CE) for each platform and ganglioside.
Analyzed ganglioside profiles in post-mortem human brain tissue extracts.

Main Results:

PRM demonstrated superior signal-to-noise ratio (up to 4-fold) and reduced variability (%RSD) through transition summation.
PRM provided richer fragmentation data (HCD) for enhanced structural elucidation, including cross-ring cleavages.
PRM enabled quantification of GM1, which was outside the mass range of the triple quadrupole.
PRM successfully distinguished GD1a and GD1b isomers in human brain tissue.

Conclusions:

PRM is a highly sensitive and structurally informative platform for ganglioside profiling.
PRM offers significant advantages over MRM for complex ganglioside analysis in biological matrices.
The optimized PRM workflow enhances ganglioside quantification accuracy and structural confidence.