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Updated: Apr 25, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Defect 3D reconstruction with integrated bright-field and dark-field structured illumination microscopy based on
This study introduces a new 3D inspection method for micro-to-nanoscale surface defects in optical components using bright-field and dark-field structured illumination microscopy (BDSIM) and deep learning. The Att-PU-Net model achieves high accuracy in reconstructing complex optical surface defects.
Area of Science:
- Optical Engineering
- Metrology
- Computer Vision
Background:
- Quantitative 3D inspection of micro-to-nanoscale surface defects in optical components for high-energy laser systems presents significant challenges.
- Existing methods struggle with sparse point clouds and noise in imaging techniques like bright-field and dark-field structured illumination microscopy (BDSIM).
Purpose of the Study:
- To develop a novel inspection framework for high-precision 3D reconstruction of micro-to-nanoscale surface defects.
- To address limitations of low luminous flux, sparse data, and noise in BDSIM imaging.
Main Methods:
- Integration of bright-field and dark-field structured illumination microscopy (BDSIM) with deep learning-based 3D reconstruction.
- Development of the Att-PU-Net model, an enhanced point cloud upsampling network (PU-Net) incorporating self-attention and multi-scale feature fusion.
- Implementation of a hybrid training strategy with simulated and real-world defect data.
- Design of a composite loss function including chamfer distance, repulsion loss, and curvature consistency.
Main Results:
- Att-PU-Net demonstrated superior geometric accuracy and uniformity compared to marching cubes and contour filter algorithms (P2S: 0.5720 µm, NUC: 0.3230).
- Experimental validation showed a reconstruction accuracy of 0.6343 µm and a maximum depth error of 0.79 µm compared to white light interferometry (WLI).
Conclusions:
- The proposed BDSIM and Att-PU-Net framework provides an effective and reliable solution for high-precision 3D reconstruction of complex optical surface defects.
- The method overcomes limitations of traditional techniques, enabling accurate defect characterization in critical optical components.
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