Phenotype recognition of pyocyanine mutants in pseudomonas aeruginosa
Journal of Bacteriology
|January 1, 1974
Abstract:
Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.


