Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.2K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adult-neurogenesis allows for representational stability and flexibility in early olfactory system.

eLife·2026
Same author

CK2 inhibition suppresses glial inflammation in models of neuroinflammation and neurodegeneration.

Nature communications·2026
Same author

Projection-specific intersectional optogenetics for precise excitation and inhibition in the marmoset brain.

Cell reports methods·2026
Same author

Opioid-specific brain connectivity dynamics distinguish analgesia from secondary effects: Studies in male mice.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Human AUTS2 regulates neurodevelopmental pathways via dual DNA/RNA binding.

bioRxiv : the preprint server for biology·2025
Same author

CK2 inhibition suppresses glial inflammation in the brain.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Apr 26, 2026

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells
08:52

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Published on: July 24, 2019

6.0K

Cell Density Impacts Population Activity in Human iPSC-Derived Neural Networks.

Yavuz Selim Uzun1,2, Renata Santos3,4, Maria C Marchetto5

  • 1Department of Physics and Astronomy, University of Rochester, Rochester, New York 14642.

Eneuro
|April 24, 2026
PubMed
Summary
This summary is machine-generated.

Culture density significantly impacts neuronal network activity in human induced pluripotent stem cell (iPSC)-derived cultures. Higher densities decrease population activity complexity, influencing network function and disease modeling.

Keywords:
MEAiPSC neural culturesnetwork sizepopulation coding

More Related Videos

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
09:20

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Published on: January 8, 2017

30.3K
Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
10:53

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Published on: November 9, 2020

4.8K

Related Experiment Videos

Last Updated: Apr 26, 2026

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells
08:52

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Published on: July 24, 2019

6.0K
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
09:20

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Published on: January 8, 2017

30.3K
Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
10:53

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Published on: November 9, 2020

4.8K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Systems Biology

Background:

  • Human induced pluripotent stem cell (iPSC)-derived neuronal cultures are valuable tools for studying network function and disease.
  • Cell culture density is a highly variable parameter across studies, with poorly understood effects on neuronal activity.
  • Understanding density's impact is crucial for reproducible and interpretable results in iPSC-based neuroscience research.

Purpose of the Study:

  • To investigate the impact of varying cell culture densities on the population activity of human iPSC-derived neuronal networks.
  • To determine how neuronal network properties change across a range of cell densities.
  • To establish an analytical framework for studying neuronal population activity in iPSC cultures.

Main Methods:

  • Multi-electrode array (MEA) recordings were performed on human iPSC-derived neuronal cultures at different densities.
  • Analysis included mean firing rates, pair-wise correlations, and population entropy.
  • A maximum entropy model was employed to analyze network activity structure.

Main Results:

  • Neuronal culture density significantly altered key network activity properties, including firing rates, correlations, and population entropy.
  • The maximum entropy model best captured network activity structure at higher culture densities.
  • Increased cellular density led to decreased complexity in population activity patterns.

Conclusions:

  • Culture density is a critical experimental parameter influencing neuronal activity and network structure in human iPSC-derived cultures.
  • Findings provide a framework for analyzing population activity, essential for disease modeling and patient-derived neuronal studies.
  • Density-dependent changes in network activity must be considered for accurate interpretation of iPSC-based neuronal culture experiments.