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PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities
Ryan Fink1,2, Shosei Imai3, Nala Gockel4
1Neuromodulatory Circuits, Institute of Zoology, University of Cologne, Cologne, Germany.
Nature Methods
|April 24, 2026
Summary
Researchers developed PinkyCaMP, a novel red genetically encoded calcium indicator (GECI). This new sensor offers improved brightness and signal quality, overcoming limitations of existing red GECIs for neuroscience research.
Area of Science:
- Neuroscience
- Biochemistry
- Optical Imaging
Background:
- Genetically encoded calcium indicators (GECIs) are crucial for observing neuronal activity.
- Existing red fluorescent GECIs often suffer from low brightness, poor signal-to-noise ratios, and photoswitching issues.
- These limitations hinder their application in advanced imaging techniques like all-optical experiments.
Purpose of the Study:
- To develop a superior red fluorescent GECI with enhanced performance.
- To create a calcium sensor compatible with blue-light optogenetics and multicolor imaging.
- To assess the sensor's efficacy and safety in various imaging modalities.
Main Methods:
- Development of PinkyCaMP, a GECI utilizing the mScarlet fluorescent protein.
- In vitro and in vivo testing of PinkyCaMP's brightness, photostability, and signal-to-noise ratio.
- Evaluation of PinkyCaMP's compatibility with blue-light optogenetics and diverse imaging techniques (fiber photometry, two-photon imaging).
Main Results:
- PinkyCaMP demonstrates significantly improved brightness and signal-to-noise ratio compared to existing red GECIs.
- The sensor shows excellent photostability and is compatible with blue-light optogenetics and multicolor imaging.
- PinkyCaMP exhibits no detectable toxicity or aggregation in neurons, both in vitro and in vivo.
Conclusions:
- PinkyCaMP represents a significant advancement in red GECI technology.
- Its enhanced properties and broad compatibility make it suitable for a wide range of neuroimaging applications.
- PinkyCaMP facilitates advanced optical neuroscience research, including in vivo imaging in awake animals.
