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Updated: Apr 26, 2026
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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
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Expanding the Analytical Toolbox for Extracellular Vesicle Biochemical Profiling: A Multiplatform Spectroscopic and
Caterina Branca1, Angela Paterna2, Estella Rao2
1Department of Mathematical and Computational Sciences, Physical Science and Earth Science, University of Messina, 98166 Messina, Italy.
Analytical Chemistry
|April 24, 2026
Summary
This study introduces a new workflow to analyze extracellular vesicle (EV) lipids using multiple techniques. It identifies phosphatidylserine as a common EV lipid signature and highlights microalgal EVs for nanobiotechnology.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Nanotechnology
Background:
- Characterizing extracellular vesicle (EV) lipid components is analytically challenging.
- Lipids are crucial for EV stability, biogenesis, and biological activity.
Purpose of the Study:
- To develop and validate an integrated workflow for comprehensive EV lipid profiling.
- To compare lipid profiles of mammalian and microalgal EVs.
- To identify conserved and species-specific lipid signatures in EVs.
Main Methods:
- Integrated workflow combining HPLC-DAD, ATR-FTIR, and GC-MS.
- Quantitative phospholipid analysis.
- Fatty acid profiling.
- Spectroscopic indicator derivation for EV source identification.
Main Results:
- Phosphatidylserine identified as a conserved, enriched lipid signature in EVs across species.
- System-specific differences in acyl chain saturation observed.
- FTIR-derived spectroscopic indicators correlate with EV source.
- Distinctive lipid and spectroscopic signatures found in microalgal EVs.
Conclusions:
- The multitechnique approach provides comprehensive structural readouts for EV identity and function.
- Microalgal EVs exhibit unique signatures, suggesting potential for nanobiotechnology.
- This workflow enhances the molecular characterization of extracellular vesicles.
