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Updated: Apr 26, 2026
Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
Published on: June 30, 2011
Role of Advanced Glycation End-Products in Altering Cellular Homeostasis and Suppressing Developmental Cycle in
Bismita Nayak1, Swastik Arya1, Sarbani Ashe1
1Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology Rourkela, Odisha, India.
Abstract:
Advanced glycation end-products (AGEs) accumulate during hyperglycemia and contribute to metabolic and neurodegenerative complications. This study investigates the effects of AGEs on Drosophila melanogaster by evaluating locomotion, oxidative stress, DNA damage, and metabolic alterations. Flies subjected to an AGEs-enriched diet exhibited erratic movement patterns, indicating neuromuscular dysfunction. Increased oxidative stress markers were observed, accompanied by significant DNA damage and disruptions in cell cycle progression, indicating genotoxic stress and the induction of apoptosis. Metabolic assessment revealed disrupted glucose and lipid homeostasis, suggesting insulin resistance and metabolic reprogramming. These findings reinforce D. melanogaster as an effective model for studying AGEs-induced metabolic and neurodegenerative dysfunctions. Understanding the pathways involved in AGEs-mediated toxicity could provide insights into AGEs-related pathologies and potential therapeutic interventions.
