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Published on: May 28, 2015
Generation of an IL2 knock-in human induced pluripotent stem cell line by CRISPR/Cas9 system
Feng Yao1, Zhai Ziqing2, Huang Jingxi2
1Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Institute for Development and Regeneration, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Interleukin-2 (IL2) is a critical cytokine that drives T-cell proliferation, activates NK cells, and holds significant therapeutic value. Its established clinical importance in cancer immunotherapy lies in sustaining the expansion, persistence, and effector function of adoptive cell therapies. Here, we established a stable IL2 knock-in human induced pluripotent stem cell (iPSC) line via CRISPR/Cas9 technology. This engineered line constitutively secretes IL2, maintains a normal karyotype, and retains typical pluripotent characteristics, including gene expression and in vivo differentiation potential. It thus provides a reliable model for studying IL2 signaling, immune crosstalk, and therapeutic screening.
Interleukin-2 (IL2) is a critical cytokine that drives T-cell proliferation, activates NK cells, and holds significant therapeutic value. Its established clinical importance in cancer immunotherapy lies in sustaining the expansion, persistence, and effector function of adoptive cell therapies. Here, we established a stable IL2 knock-in human induced pluripotent stem cell (iPSC) line via CRISPR/Cas9 technology. This engineered line constitutively secretes IL2, maintains a normal karyotype, and retains typical pluripotent characteristics, including gene expression and in vivo differentiation potential. It thus provides a reliable model for studying IL2 signaling, immune crosstalk, and therapeutic screening.
