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Mesoporous silica nanoparticles encapsulating xylanase: Efficient platform for low-polymerization-degree
Xuefei Zhu1, Aitao Li2, Caoxing Huang1
1State Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing 210037, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Products, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Immobilizing xylanase on mesoporous silica nanoparticles (MSN) enhances xylooligosaccharides (XOS) production. This method improves enzyme stability and yields bioactive XOS fractions (X2-X3) with high efficiency and reusability.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Carbohydrate Chemistry
Background:
- Xylooligosaccharides (XOS) are valuable prebiotics with significant biological activity.
- Efficient production of XOS with controlled degree of polymerization (DP) is crucial for maximizing bioactivity.
- Industrial XOS production is hindered by the instability of free xylanase enzymes.
Purpose of the Study:
- To develop a stable and reusable xylanase catalyst for efficient XOS production.
- To investigate the use of mesoporous silica nanoparticles (MSN) for xylanase immobilization.
- To optimize the production of low DP XOS with enhanced biological activity.
Main Methods:
- Xylanase enzyme was immobilized onto mesoporous silica nanoparticles (MSN) to create Xyl@MSN.
- The catalytic performance of Xyl@MSN was evaluated for XOS production from xylan.
- Enzyme activity, XOS yield, DP distribution, and reusability were assessed.
Main Results:
- Xyl@MSN achieved a high yield of XOS with DP ranging from 2 to 6.
- Recovered enzyme activity was 76.85%, with residual activity 37.7% higher than free enzyme.
- Conversion to X2-X6 reached 66.90%, with products predominantly X2 and X3.
- The immobilized enzyme retained 41% activity after five cycles, with a half-life of 462.19 min.
Conclusions:
- Mesoporous silica nanoparticle immobilization significantly enhances xylanase stability and reusability.
- This approach enables efficient production of highly bioactive XOS, primarily X2 and X3.
- The developed Xyl@MSN catalyst offers a promising technology for industrial XOS synthesis.
