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Development of a HiFi-LAMP assay for the detection of herpes simplex virus type 1

Ziyan Wang1,2, Zhenzhou Wan3, Yongjuan Zhao4

  • 1School of Medicine, Anhui University of Science and Technology, Huainan, 232001, China.

Scientific Reports
|April 26, 2026
PubMed
Abstract

No abstract available in PubMed .

Keywords:
HSV-1HiFi-LAMPIsothermal amplificationMolecular diagnosticsPOCT

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Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
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Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...

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