Related Experiment Video
Updated: Apr 28, 2026
08:30
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
27.7K
Comparative diagnostic performance of plasma microbial cell-free DNA sequencing versus amplicon-based blood
Mayar Al Mohajer1,2, Todd Lasco3
1Nuffield Department of Primary Health Care and Science, University of Oxford, Oxford, UK.
Antimicrobial Stewardship & Healthcare Epidemiology : ASHE
|April 27, 2026
Abstract:
We compared plasma microbial cell-free DNA sequencing (mcfDNAseq) with an amplicon-based blood next-generation sequencing (NGS) assay for 10 patients with suspected bloodstream or endovascular infection. Plasma testing detected pathogens in eight (seven clinically meaningful), whereas amplicon testing was negative in all. Plasma mcfDNAseq provided higher test yield and overall sensitivity.
Related Concept Videos
01:29Modern Molecular Taxonomy
833
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
833
01:24Automated Microbial Diagnostics
68
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
68