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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Apr 28, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Detection of a sequence feature for recursive splicing.

Bixuan Wang^1,2,3,4, Kevin Yang^5,6,7, Yoseph Barash^5,8

¹Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA.

Biorxiv : the Preprint Server for Biology

|April 27, 2026

Summary

This summary is machine-generated.

Recursive splicing, a process where spliceosomes remove introns in segments, is more common in first introns. Specific CG-rich sequence motifs near the first intron influence recursive splicing throughout the RNA transcript.

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Last Updated: Apr 28, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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Published on: December 9, 2016

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Published on: September 15, 2021

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Molecular Biology
Genetics
RNA Processing

Background:

RNA splicing removes introns from pre-mRNA to form mature mRNA.
Recursive splicing is a mechanism where large introns are removed in segments by the spliceosome.
The sequence elements governing recursive splicing have remained largely unidentified.

Purpose of the Study:

To identify cis-acting sequence signals associated with recursive splicing.
To investigate the influence of first introns on recursive splicing patterns.
To develop a predictive model for recursive splicing.

Main Methods:

Probabilistic mixture models were employed to analyze sequence data.
Sequence features flanking the first intron were examined.
A novel medium-throughput primer extension assay was utilized for validation.

Main Results:

Recursive splicing is more frequent in longer, CG-rich first introns with specific flanking sequences.
Depletion of CGs in the downstream polypyrimidine tract was observed.
First intron sequence properties predict recursive splicing frequency in downstream introns.
A sequence-dependent classifier for recursive splicing was developed and validated.

Conclusions:

Newly identified sequence motifs at the beginning of RNA synthesis influence recursive splicing.
Early RNA processing events, dictated by first intron sequences, affect subsequent splicing.
The findings provide insights into the regulation of recursive splicing.