Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

3D Passive Cavitation Mapping (3D-PCM) with a Large-aperture Planar Array.

bioRxiv : the preprint server for biology·2026

Same author

Therapeutic potential of modulating endogenous PYY expression for controlling overweight and obesity: a narrative review.

Frontiers in nutrition·2026

Same author

Noninvasive whole-brain imaging of glymphatic dynamics.

Science advances·2026

Same author

Posterior unilateral approach with unilateral pedicle and contralateral laminar screw combined with lateral mass reconstruction for cervical dumbbell tumors: case series and technical note.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026

Same author

RNA binding protein YWHAZ mediates specific mRNA translation and regulates cell proliferation and apoptosis in diabetic foot ulcer.

Frontiers in medicine·2026

Same author

Hepatocyte hedgehog signaling controls ferroptosis to alleviate aging-related organ dysfunction.

JCI insight·2026

Search research articles

Related Experiment Video

Updated: May 5, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Photoacoustic Fingerprinting for Robust Molecular Imaging.

Colton McGarraugh¹, Luca Menozzi¹, Rui Yao¹

¹Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.

Biorxiv : the Preprint Server for Biology

|April 27, 2026

Summary

Photoacoustic fingerprinting (PAF) enhances molecular imaging by treating spectral unmixing as a fingerprint recognition problem. This AI-driven approach offers robust quantification, overcoming limitations of conventional methods in complex biological samples.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

Related Experiment Videos

Last Updated: May 5, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

Area of Science:

Biomedical Imaging
Molecular Imaging
Photoacoustics

Background:

Quantitative molecular imaging in photoacoustics is challenged by spectral unmixing limitations.
Conventional methods suffer from spectral overlap, noise, and unknown fluence, introducing bias.

Purpose of the Study:

Introduce photoacoustic fingerprinting (PAF) to reframe spectral unmixing as a fingerprint recognition problem.
Develop a robust framework for accurate molecular quantification in photoacoustic imaging.

Main Methods:

Interpreted multispectral signals as high-dimensional fingerprints encoding molecular composition and distortions.
Utilized a recurrent neural network trained on synthetic data with realistic variations.
Applied PAF to simulations, phantom studies, ex vivo mouse livers, and human arteries.

Main Results:

PAF demonstrated accurate and robust quantification, outperforming non-negative least squares, especially for spectrally overlapping chromophores.
Improved molecular specificity in phantom studies, correctly localizing collagen and recovering water contrast.
Detected lipid accumulation in steatotic livers and identified molecular signatures in human arteries.

Conclusions:

PAF is a generalizable framework for label-free molecular imaging.
Represents a significant advancement toward quantitative photoacoustic diagnostics.