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Quantifying Mg2+ dependence on conformational equilibrium in the two-state 7SK RNA stem-loop 3

Shilla Owusu Ansah1, Momodou B Camara1, Catherine D Eichhorn1,2

  • 1Department of Chemistry, University of Nebraska - Lincoln, 639 North 12 St, Lincoln, NE 68588, USA.

Biorxiv : the Preprint Server for Biology
|April 27, 2026
PubMed
Summary

Magnesium ions (Mg2+) alter the structural equilibrium of 7SK RNA's SL3 domain by favoring the SL3e conformer. This study provides a thermodynamic framework for understanding ion-dependent RNA dynamics.

Keywords:
RNA dynamicschemical probingconformational exchangeion-RNA interactionsisothermal titration calorimetrylinkage equilibriumsolution NMR spectroscopy

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