Optimizing and tailoring cold atmospheric plasma parameters for C. albicans biofilms eradication

Area of Science:

• Microbiology
• Biophysics
• Biotechnology

Background:

• Candida albicans biofilms pose significant challenges in healthcare settings due to their resistance to conventional therapies.
• Understanding the mechanisms of biofilm control is crucial for developing effective treatment strategies.

Purpose of the Study:

• To investigate the efficacy of cold atmospheric plasma (CAP) in eradicating Candida albicans biofilms.
• To determine the influence of applied voltage and treatment duration on CAP's effectiveness against biofilms.

Main Methods:

• Evaluation of CAP effects on inhibition zones, biofilm viability, metabolism, and cell membrane integrity.
• Assessment of biofilm oxidative stress, biomass composition (lipids, proteins, carbohydrates), and hyphal structure.
• Correlation of CAP treatment parameters (voltage, duration) with observed biofilm changes.

Main Results:

• Increased applied voltage and treatment duration significantly reduced biofilm viability, with complete eradication achieved at higher parameters.
• CAP treatment led to increased cell membrane damage and oxidative stress within the biofilms.
• Morphological analysis revealed hyphal contraction and compression, alongside significant alterations in biofilm biomass composition.

Conclusions:

• Optimal cold atmospheric plasma (CAP) parameters demonstrate significant potential for controlling Candida albicans biofilms.
• CAP treatment induces complex changes in biofilm viability, morphology, and composition, offering a promising therapeutic avenue.