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Investigation of Biosubjects with Diffuse Reflectance Spectroscopy.

Mei-Yu Hsu1, Cheng-Yoong Pang2, Shinn-Zong Lin2

  • 1Department of Nursing, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 707, Sec. 3, Chung-Yang Rd., Hualien City 970, Taiwan.

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Summary
This summary is machine-generated.

Diffuse reflectance spectroscopy (DRS) with visible and near-infrared (NIR) spectrometers can identify unique biological samples. NIR absorption with DRS effectively monitors fruit dehydration and human skin hydration.

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Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Healthcare Technology

Background:

  • Diffuse reflectance spectroscopy (DRS) is a valuable technique for analyzing biological samples.
  • Visible and near-infrared (NIR) spectrometers offer complementary spectral information.
  • Understanding hydration and dehydration processes is crucial in biological and medical contexts.

Purpose of the Study:

  • To develop and utilize a portable DRS apparatus for investigating biological subjects.
  • To assess the capability of visible and NIR DRS in characterizing various biological products.
  • To evaluate the potential of NIR absorption with DRS for monitoring oxidation, dehydration, and hydration levels.

Main Methods:

  • Assembly of a portable spectrometric apparatus with visible and NIR spectrometers.
  • Measurement of direct visible DRS for diverse fruits and peppers.
  • Monitoring of apple slice oxidation and dehydration using NIR absorption with DRS.
  • Assessment of human skin hydration levels using NIR absorption with DRS after mask application.

Main Results:

  • Each biological item exhibited a distinctive visible DRS curve.
  • Visible DRS analysis of time-dependent changes in apple slices was challenging.
  • NIR absorption with DRS successfully monitored apple slice dehydration stages.
  • NIR absorption with DRS effectively monitored human skin hydration levels.

Conclusions:

  • DRS is a versatile tool for characterizing various biological products.
  • NIR absorption with DRS is effective for monitoring dehydration and hydration in biological samples.
  • DRS shows significant promise for applications in biochemistry, medical diagnosis, and healthcare.