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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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The Multi-Attribute Method (MAM), An Advanced LC-MS Approach for Protein A Resin Performance and Lifecycle

Jingming Zhang1, Matthew Larsen2, Timothy Blanc3

  • 1Analytical Sciences, Eli Lilly and Company, Branchburg, NJ 08876, USA.

Antibodies (Basel, Switzerland)
|April 27, 2026
PubMed
Summary
This summary is machine-generated.

The multi-attribute method (MAM) now offers a unified LC-MS platform for analyzing Protein A resins, assessing ligand integrity and detecting impurities. This provides a molecular-level understanding for improved resin lifecycle management in antibody production.

Keywords:
Protein A resinsclean-in-place (CIP)host cell proteins (HCPs)liquid chromatography–mass spectrometry (LC-MS)multi-attribute method (MAM)peptide mappingpost-translational modifications (PTMs)resin fouling

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Analytical Chemistry
  • Chromatography

Background:

  • Protein A resins are critical for monoclonal antibody (mAb) production but lack direct, quantitative assessment methods.
  • Current methods for evaluating Protein A resin condition are often indirect or qualitative.

Purpose of the Study:

  • To adapt the multi-attribute method (MAM) as a unified liquid chromatography-mass spectrometry (LC-MS) platform for Protein A resin analysis.
  • To establish a comprehensive strategy for Protein A resin lifecycle management.

Main Methods:

  • Systematic adaptation of MAM for Protein A resin analysis using LC-MS.
  • Evaluation of four Cytiva Protein A resins (MabSelect™, MabSelect SuRe™, MabSelect SuRe™ LX, MabSelect™ PrismA).
  • Assessment of resin identity, Protein A ligand integrity, fouling, and cleaning performance.

Main Results:

  • MAM enabled resin-specific peptide fingerprinting and quantitative monitoring of Protein A ligand post-translational modifications (PTMs).
  • Analysis revealed ligand degradation and fouling in used resins, with MabSelect™ being most susceptible.
  • Direct detection and quantification of residual mAbs and host cell proteins (HCPs) from the resin matrix were achieved.

Conclusions:

  • MAM is established as a novel, sensitive, and comprehensive strategy for Protein A resin lifecycle management.
  • The method provides actionable insights for resin selection, cleaning optimization, and downstream process development.
  • MAM offers molecular-level assessment of resin cleaning effectiveness, surpassing conventional approaches.