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Fluorescence Immunosensor with Phage Antibodies for Heat Shock Protein 70 Detection
Olga I Guliy1, Sergei A Eremin2, Liliya I Mukhametova2
1Institute of Biochemistry and Physiology of Plants and Microorganisms-Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia.
Biosensors
|April 27, 2026
Summary
This study introduces a new fluorescence immunoassay for detecting heat shock proteins, crucial cancer biomarkers. This method offers rapid, sensitive detection, aiding in early cancer diagnosis and treatment monitoring.
Area of Science:
- Biochemistry
- Immunology
- Cancer Diagnostics
Background:
- Early cancer diagnosis and tailored antitumor therapy rely on detecting specific biological markers.
- Real-time, sensitive, and selective monitoring of biomarkers is essential for point-of-care diagnostics.
Purpose of the Study:
- To develop and validate a fluorescence immunoassay for detecting heat shock proteins (HSPs) as cancer biomarkers.
- To establish the sensitivity and reliability of the developed immunoassay.
Main Methods:
- Detection of heat shock proteins using fluorescence immunoassay with specific phage antibodies.
- Confirmation of results using dot immunoassay and circular dichroism spectroscopy.
Main Results:
- The fluorescence immunoassay achieved a minimum detection limit of 1 ng/mL for heat shock proteins.
- Assay data were corroborated by dot immunoassay and circular dichroism studies, confirming reliability.
Conclusions:
- The developed fluorescence immunoassay demonstrates high sensitivity and selectivity for detecting heat shock proteins.
- This method shows potential for adaptation in point-of-care cancer diagnostics and monitoring antitumor therapy.
