Cellulose-Based Hydrogels Incorporating Olive Mill Wastewater (OMW): Preparation, Characterization, and In Vitro...

Area of Science:

• Biomaterials Science
• Dermatology
• Sustainable Chemistry

Background:

• Olive mill wastewater (OMW) is a rich source of bioactive polyphenols with antioxidant and antimicrobial potential.
• Valorization of OMW is crucial for sustainable agro-industrial practices.
• Cellulose-based hydrogels offer biocompatible platforms for topical delivery.

Purpose of the Study:

• To incorporate purified OMW fractions (RO1, MD2) into cellulose-based hydrogels for topical applications.
• To evaluate the physicochemical, antioxidant, and antimicrobial properties of OMW-loaded hydrogels.
• To assess the potential of these hydrogels for dermatological applications.

Main Methods:

• Purified OMW fractions (RO1, MD2) were incorporated at 10% (w/w) into hydrogels made from carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), hydroxypropyl methylcellulose (HPMC), and methylcellulose (MC).
• Formulations were characterized for organoleptic properties, pH, rheology, swelling, weight loss, antioxidant activity (DPPH assay), and antimicrobial efficacy against skin pathogens.
• Rheological analysis was performed to determine suitability for topical administration.

Main Results:

• OMW-loaded hydrogels exhibited pseudoplastic rheological behavior, suitable for topical application.
• Significant radical scavenging activity was observed in OMW-loaded hydrogels compared to controls.
• Antimicrobial efficacy against selected skin pathogens, including antibiotic-resistant strains, was demonstrated, confirming preserved OMW bioactivity.

Conclusions:

• Cellulose-based hydrogels can effectively encapsulate and preserve the bioactivity of OMW polyphenols.
• OMW-loaded hydrogels show promise as sustainable and biocompatible carriers for dermatological applications.
• These formulations could be beneficial for managing oxidative stress and bacterial skin infections.