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Related Experiment Video

Updated: Apr 28, 2026

Lipidomics and Transcriptomics in Neurological Diseases
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Lipidomics and Transcriptomics in Neurological Diseases

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Optimized Protocols to Extract Total Transcripts and Proteins from Lipid-Rich Tissues.

Nicolas De Azevedo1, Anthony Lozano1,2,3, Ramon E Parsons1,4

  • 1Tisch Cancer Institute, Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Methods and Protocols
|April 27, 2026
PubMed
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Extracting RNA and protein from lipid-rich tissues like adipose tissue can be difficult. This study identifies optimized TRIzol and kit-based methods for improved yields in challenging samples.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Highly lipidic tissues present significant challenges for molecular extraction and analysis.
  • Lipids interfere with various laboratory processes, including filtration, sequencing, imaging, and separation.

Purpose of the Study:

  • To determine optimal methods for extracting total RNA for RT-qPCR.
  • To identify effective methods for total protein extraction and quantification.
  • To compare extraction efficiencies in mice on regular versus high-fat diets.

Main Methods:

  • Comparison of three distinct total RNA extraction protocols.
  • Evaluation of two different total protein extraction methods.
  • Utilized TRIzol, chloroform, and a specialized adipose tissue kit.
Keywords:
RNARNA-seqRT-qPCRadiposebrainlipid-rich tissuesliverproteintranscript

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Related Experiment Videos

Last Updated: Apr 28, 2026

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Main Results:

  • TRIzol combined with chloroform yielded the highest total RNA quantity and purity, with protocol optimizations for lipid-rich samples.
  • An adipose tissue-specific kit outperformed the RIPA-based method for total protein extraction.
  • Adipose tissue proved more challenging to process than brain and liver tissues.

Conclusions:

  • Adipose tissue, especially when subjected to a high-fat diet, is the most difficult lipid-rich tissue for molecular extraction.
  • Optimized protocols enhance RNA and protein yields and purity from challenging tissues.
  • Findings offer valuable insights for researchers working with lipid-rich samples.