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Polygoni Multiflori Radix Improves Dyslipidemia by Regulating Hepatocyte Lipid Metabolism Mediated via the
Tongye Wang1, Lijuan Zhang1,2, Yue Wang1
1Xinjiang Key Laboratory of Uyghur Medical Research, Xinjiang Institute of Materia Medica, Urumqi 830004, China.
Objective:
To clarify the molecular mechanism of Polygoni Multiflori Radix in improving dyslipidemia and provide a scientific basis for its clinical application.
Methods:
Network pharmacology, molecular docking and simulation were used to predict and verify active components and core targets of Polygoni Multiflori Radix. HFD-induced hyperlipidemic mice were grouped and administered for 28 days; serum indices, hepatic pathology and AMPK/SREBP-2/PCSK9/LDLR pathway expression were detected.
Results:
Twenty-two active components, 101 potential targets and the AMPK pathway (core) were identified. TSG, its main component, bound stably to AMPK/SREBP-2/PCSK9/LDLR. Polygoni Multiflori Radix dose-dependently improved HFD-induced abnormalities in mice (p < 0.05 or p < 0.01).
Conclusions:
Polygoni Multiflori Radix effectively improves HFD-induced dyslipidemia by regulating the AMPK/SREBP-2/PCSK9/LDLR pathway to ameliorate hepatocyte lipid metabolism and reduce oxidative stress/liver injury.
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